mouse gecko v2 library b Search Results


mouse geckov2 library b  (Addgene inc)
93
Addgene inc mouse geckov2 library b
Mouse Geckov2 Library B, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+gecko+v2+library+b/pmc12041282-237-1-4?v=Addgene+inc
Average 93 stars, based on 1 article reviews
mouse geckov2 library b - by Bioz Stars, 2026-07
93/100 stars
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mouse gecko v2 library b  (Addgene inc)
93
Addgene inc mouse gecko v2 library b
Fig. 2 | CRISPR–Cas9 screening of primary mouse macrophages to identify TAM regulators. a, Experimental scheme of genome-wide CRISPR–Cas9 Knockout <t>GecKO</t> <t>v2</t> Library B screening in primary murine macrophages. b, Representative plot of backbone (LGP) or library-B-infected macrophages exposed or not to conditioned media from Pten−/−Trp53−/− tumor cells. c, Two independent experiments were performed. Graphs show the correlations between the distribution of the guides found in the CD206−MHCII+ population (MHCII) and in the CD206brightMHCII− population (CD206) from the two experiments. Lib1, library 1; Lib2, library 2. d, Volcano plot showing genes related to the differentially enriched sgRNA guides from CD206−MHCII+ versus CD206brightMHCII− cells. Negative regulators of the CD206brightMHCII− population are shown in light blue, and positive regulators are shown in red. log2FC ± 0.56, P < 0.005. Statistical analyses and comparisons from NGS output
Mouse Gecko V2 Library B, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+gecko+v2+library+b/pm40588561-540-1-6?v=Addgene+inc
Average 93 stars, based on 1 article reviews
mouse gecko v2 library b - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier

Image Search Results


Fig. 2 | CRISPR–Cas9 screening of primary mouse macrophages to identify TAM regulators. a, Experimental scheme of genome-wide CRISPR–Cas9 Knockout GecKO v2 Library B screening in primary murine macrophages. b, Representative plot of backbone (LGP) or library-B-infected macrophages exposed or not to conditioned media from Pten−/−Trp53−/− tumor cells. c, Two independent experiments were performed. Graphs show the correlations between the distribution of the guides found in the CD206−MHCII+ population (MHCII) and in the CD206brightMHCII− population (CD206) from the two experiments. Lib1, library 1; Lib2, library 2. d, Volcano plot showing genes related to the differentially enriched sgRNA guides from CD206−MHCII+ versus CD206brightMHCII− cells. Negative regulators of the CD206brightMHCII− population are shown in light blue, and positive regulators are shown in red. log2FC ± 0.56, P < 0.005. Statistical analyses and comparisons from NGS output

Journal: Nature immunology

Article Title: Chemosensor receptors are lipid-detecting regulators of macrophage function in cancer.

doi: 10.1038/s41590-025-02191-x

Figure Lengend Snippet: Fig. 2 | CRISPR–Cas9 screening of primary mouse macrophages to identify TAM regulators. a, Experimental scheme of genome-wide CRISPR–Cas9 Knockout GecKO v2 Library B screening in primary murine macrophages. b, Representative plot of backbone (LGP) or library-B-infected macrophages exposed or not to conditioned media from Pten−/−Trp53−/− tumor cells. c, Two independent experiments were performed. Graphs show the correlations between the distribution of the guides found in the CD206−MHCII+ population (MHCII) and in the CD206brightMHCII− population (CD206) from the two experiments. Lib1, library 1; Lib2, library 2. d, Volcano plot showing genes related to the differentially enriched sgRNA guides from CD206−MHCII+ versus CD206brightMHCII− cells. Negative regulators of the CD206brightMHCII− population are shown in light blue, and positive regulators are shown in red. log2FC ± 0.56, P < 0.005. Statistical analyses and comparisons from NGS output

Article Snippet: The mouse GeCKO v2 Library B (Addgene) was used: this library consists of 62,804 sgRNAs constructs, with three sgRNAs targeting each of the 20,661 genes of the mouse genome.

Techniques: CRISPR, Genome Wide, Knock-Out, Infection